Abstract
The biocide chlorhexidine (CHX) as well as additional membrane-active agents were shown to induce expression of the mexCD-oprJ multidrug efflux operon, dependent upon the AlgU stress response sigma factor. Hyperexpression of this efflux system in nfxB mutants was also substantially AlgU dependent. CHX resistance correlated with efflux gene expression in various mutants, consistent with MexCD-OprJ being a determinant of CHX resistance.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Infective Agents, Local / pharmacology*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Cell Membrane / drug effects
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Chlorhexidine / pharmacology*
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Drug Resistance, Bacterial*
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Gene Expression Regulation, Bacterial*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism
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Microbial Sensitivity Tests
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Operon
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Pseudomonas aeruginosa / drug effects*
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / metabolism
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Sigma Factor / genetics
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Sigma Factor / metabolism
Substances
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AlgU protein, Pseudomonas aeruginosa
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Anti-Infective Agents, Local
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Bacterial Proteins
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Membrane Proteins
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Membrane Transport Proteins
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MexC protein, Pseudomonas aeruginosa
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MexD protein, Pseudomonas aeruginosa
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OprJ protein, Pseudomonas aeruginosa
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Sigma Factor
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Chlorhexidine