Abstract
In this study, the relationship between the dCTP/ara-CTP ratio and the cytotoxic effect of cytosine arabinoside (ara-C) was investigated. Intracellular levels of dNTPs and ara-CTP were analyzed by high-performance liquid chromatography. Although doubling time and intracellular dCTP levels were different in each of the cells, there was a consistent relation between the intracellular dCTP/ara-CTP ratio and the cytotoxic concentration of ara-C. These data suggest that the intracellular dCTP/ara-CTP ratio is one of the important factors for considering the cytotoxic action of ara-C.
MeSH terms
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Arabinofuranosylcytosine Triphosphate / analysis*
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Arabinofuranosylcytosine Triphosphate / metabolism
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Cell Division / drug effects
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Chromatography, High Pressure Liquid
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Culture Media / chemistry
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Cytarabine / metabolism
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Cytarabine / pharmacology*
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DNA, Neoplasm / metabolism
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Deoxycytosine Nucleotides / analysis*
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Deoxycytosine Nucleotides / metabolism
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Humans
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Tumor Cells, Cultured / chemistry
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / metabolism
Substances
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Culture Media
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DNA, Neoplasm
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Deoxycytosine Nucleotides
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Cytarabine
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Arabinofuranosylcytosine Triphosphate
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2'-deoxycytidine 5'-triphosphate