Leiomyoma cells with 12q15 aberrations can be transformed in vitro and show a relatively stable karyotype during precrisis period

C Stern; B Kazmierczak; B Thode; B Rommel; S Bartnitzke; P Dal Cin; W van de Ven; H Van den Berghe; J Bullerdiek

doi:10.1016/0165-4608(91)90210-l

Leiomyoma cells with 12q15 aberrations can be transformed in vitro and show a relatively stable karyotype during precrisis period

Cancer Genet Cytogenet. 1991 Jul 15;54(2):223-8. doi: 10.1016/0165-4608(91)90210-l.

Authors

C Stern¹, B Kazmierczak, B Thode, B Rommel, S Bartnitzke, P Dal Cin, W van de Ven, H Van den Berghe, J Bullerdiek

Affiliation

¹ Center of Human Genetics and Genetic Counselling, University of Bremen, Fed. Republic of Germany.

PMID: 1884354
DOI: 10.1016/0165-4608(91)90210-l

Abstract

Tumor cells from three uterine leiomyomas showing translocations involving 12q14-15 were transformed by transfection using the "early regions" of the SV40 genome. The cells had a higher proliferative capacity, were able to form colonies in soft agar, and showed an increased growth potential. Karyotype analyses of these transformed leiomyoma cells showed that the cells had retained the initial t(12;14) and t(12;15).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, Polyomavirus Transforming / genetics
Blotting, Southern
Cell Transformation, Neoplastic / genetics
Cell Transformation, Neoplastic / pathology*
Chromosome Aberrations / genetics
Chromosome Aberrations / pathology*
Chromosome Banding
Chromosome Disorders
Chromosomes, Human, Pair 12*
DNA, Neoplasm / genetics
Female
Humans
Leiomyoma / genetics
Leiomyoma / pathology*
Transfection
Uterine Neoplasms / genetics
Uterine Neoplasms / pathology

Substances

Antigens, Polyomavirus Transforming
DNA, Neoplasm