Objective: To examine the APOBEC3G (hA3G) mRNA levels of four different groups in the human immunodeficiency virus (HIV) prevalent areas in central China and to analyze the relationship between hA3G mRNA levels and HIV disease progression.
Methods: We collected peripheral blood and isolated the peripheral boold monouuclear cells (PBMCs), and then cryo-preserved the PBMCs in liquid nitrogen. Prior to the total extraction of RNA, PBMCs were resuscitated and mRNA were reverse Transcripted to cDNA in vitro. Real-time polymerase chain reaction (PCR) was used to test hA3G mRNA levels of different groups.
Results: There were 13 HIV long term non-progressors with the mean CD4+ T lymphocyte count as (716 +/- 169) per microl and the mean affection time as (12.5 +/- 2.3) years. There were 48 HIV slow progressors with the mean CD4+ T lymphocyte count as (233 +/- 144) per microl and the mean affection time as (10.7 +/- 2.2) years. The hA3G mRNA level of HIV long term nonprogressors was higher than that of normal people while the hA3G mRNA level of HIV slow progressors was higher than that of normal people and high risk people. There were no correlations between CD4+ T lymphocyte count and hA3G mRNA levels of HIV long-term nonprogressors as well as in HIV slow progressors.
Conclusion: There was difference found in the hA3G mRNA levels of four groups in the HIV popular area in central China while no correlation between CD4+ T lymphocyte count and hA3G mRNA levels of HIV long term nonprogressors as well as in HIV slow progressors were found.