Abstract
The embryonic striatal temperature sensitive immortalized ST14A-cell line was characterized in vitro by immunocytochemistry when cultured at 33 degrees C and at nonpermissive temperature of 39 degrees C for up to 14 days. At 33 degrees C in DMEM/10% FCS, cells proliferated, were extensively expressing the neural progenitor cell markers nestin and vimentin contrary to neuronal markers. However, when cultured at 39 degrees C the proliferation was delayed and cells began to increase the expression of neuronal markers, followed by a decrease of nestin and vimentin. In serum-free medium the process of neuronal differentiation became more obvious, indicating the potential to use these cells for experimental restorative therapies.
MeSH terms
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Animals
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Biomarkers / metabolism
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Cell Culture Techniques
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Cell Line, Transformed
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Cell Proliferation
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Corpus Striatum / cytology*
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Corpus Striatum / embryology
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Corpus Striatum / metabolism
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Culture Media, Serum-Free / pharmacology
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Huntington Disease / therapy
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Intermediate Filament Proteins / metabolism
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Microtubule-Associated Proteins / metabolism
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Nerve Tissue Proteins / metabolism
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Nestin
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Neurogenesis / physiology*
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Rats
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Stem Cell Transplantation*
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Stem Cells / cytology
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Stem Cells / metabolism*
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Temperature
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Vimentin / metabolism
Substances
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Biomarkers
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Culture Media, Serum-Free
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Intermediate Filament Proteins
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Microtubule-Associated Proteins
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Nerve Tissue Proteins
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Nes protein, rat
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Nestin
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Vimentin
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microtubule-associated protein 1B