Abstract
Ovarian cortical fragments (3 x 3 x 1 mm) were exposed to dimethyl sulfoxide (DMSO) in different concentrations for further analysis of cryoprotectant perfusion by applying high-performance liquid chromatography (HPLC) and conventional cryopreservation. This simple perfusion test can predict the efficiency of the cryopreservation procedure.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chromatography, High Pressure Liquid / methods*
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Cryopreservation / methods*
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Cryoprotective Agents / administration & dosage
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Cryoprotective Agents / pharmacology*
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Dimethyl Sulfoxide / administration & dosage
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Dimethyl Sulfoxide / pharmacology*
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Dose-Response Relationship, Drug
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Female
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Goats
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Models, Animal
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Ovarian Follicle / drug effects*
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Ovarian Follicle / physiology
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Ovary / drug effects*
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Ovary / physiology
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Perfusion / methods
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Tissue Survival / drug effects*
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Tissue Survival / physiology
Substances
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Cryoprotective Agents
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Dimethyl Sulfoxide