Abstract
The CD11c(int)B220(+)NK1.1(+)CD49(+) subset of cells has recently been described as IFN-producing killer dendritic cells (IKDC), which share phenotypic and functional properties of dendritic cells and NK cells. Herein we show that bone marrow-derived murine dendritic cell preparations contain abundant CD11c(int)B220(+)NK1.1(+)CD49(+) cells, the removal of which results in loss of tumoricidal activity of unpulsed dendritic cells in vivo. Moreover, following s.c. injection, as few as 5 x 10(3) highly pure bone marrow-derived IKDC cells are capable of shrinking small contralateral syngeneic tumors in C57BL/6 mice, but not in immunodeficient mice, implying the obligate involvement of host effector cells in tumor rejection. Our data suggest that bone marrow-derived IKDC represent a population that has powerful tumoricidal activity in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bone Marrow Cells / immunology*
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Bone Marrow Cells / metabolism
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Cell Death / immunology
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Cell Line, Tumor
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Cells, Cultured
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Cricetinae
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Cricetulus
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Cytotoxicity, Immunologic*
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism
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Dendritic Cells / transplantation*
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Immunotherapy, Adoptive / methods
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Interferon-gamma / biosynthesis
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Interferon-gamma / metabolism
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Interferon-gamma / physiology*
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Killer Cells, Natural / immunology*
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Killer Cells, Natural / metabolism
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Lung Neoplasms / chemically induced
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Lung Neoplasms / immunology
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Lung Neoplasms / therapy
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Melanoma, Experimental / immunology
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Melanoma, Experimental / metabolism
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Melanoma, Experimental / therapy
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Neoplasms / immunology*
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Neoplasms / pathology
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Neoplasms / therapy*
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Pulmonary Alveoli / immunology
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Pulmonary Alveoli / pathology
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Rats
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Rhabdomyosarcoma / chemically induced
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Rhabdomyosarcoma / immunology
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Rhabdomyosarcoma / therapy
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T-Lymphocytes, Cytotoxic / immunology
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T-Lymphocytes, Cytotoxic / metabolism