Percent positive rate of Lyme real-time polymerase chain reaction in blood, cerebrospinal fluid, synovial fluid, and tissue

Ngolela Esther Babady; Lynne M Sloan; Emily A Vetter; Robin Patel; Matthew J Binnicker

doi:10.1016/j.diagmicrobio.2008.08.016

Percent positive rate of Lyme real-time polymerase chain reaction in blood, cerebrospinal fluid, synovial fluid, and tissue

Diagn Microbiol Infect Dis. 2008 Dec;62(4):464-6. doi: 10.1016/j.diagmicrobio.2008.08.016. Epub 2008 Oct 22.

Authors

Ngolela Esther Babady¹, Lynne M Sloan, Emily A Vetter, Robin Patel, Matthew J Binnicker

Affiliation

¹ Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.

PMID: 18947959
DOI: 10.1016/j.diagmicrobio.2008.08.016

Abstract

We performed a retrospective review of 23,777 specimens tested by our Lyme real-time polymerase chain reaction assay to determine the percent positive rates by specimen source. The percent positive rates were highest in synovial fluid (6.4%) and tissue (6.5%), and lowest in blood (0.1%) and cerebrospinal fluid (0.09%).

MeSH terms

Bacterial Proteins / analysis
Borrelia burgdorferi / isolation & purification*
Cerebrospinal Fluid / microbiology
Humans
Lyme Disease*
Polymerase Chain Reaction / methods*
Reproducibility of Results
Retrospective Studies
Synovial Fluid / microbiology

Substances

Bacterial Proteins