Very small embryonic-like stem cells are present in adult murine organs: ImageStream-based morphological analysis and distribution studies

Cytometry A. 2008 Dec;73A(12):1116-27. doi: 10.1002/cyto.a.20667.

Abstract

Recently, we purified a population of CXCR4+/Oct-4+/SSEA-1+/Sca-1+/Lin(-)/CD45(-) very small embryonic-like stem cells (VSELs) from adult murine bone marrow (BM). After using flow cytometry, ImageStream analysis, confocal microscopy, and real time RT-PCR, we report that similar cells could be also identified and isolated from several organs in adult mice. The highest total numbers of Oct-4+ VSELs were found in the brain, kidneys, muscles, pancreas, and BM. These observations support our hypothesis that a population of very primitive cells expressing germ line/epiblast markers (Oct-4, SSEA-1) is deposited early during embryogenesis in various organs and survives into adulthood. Further studies are needed to determine whether these cells, after being isolated from various adult human organs similarly to their murine BM-derived counterparts, are endowed with pluripotent stem cell properties.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ataxin-1
  • Ataxins
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism
  • Cell Separation
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / metabolism
  • Flow Cytometry
  • Lewis X Antigen / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Confocal
  • Nerve Tissue Proteins / metabolism
  • Nuclear Proteins / metabolism
  • Octamer Transcription Factor-3 / metabolism
  • RNA, Messenger / metabolism

Substances

  • ATXN1 protein, human
  • Ataxin-1
  • Ataxins
  • Atxn1 protein, mouse
  • Lewis X Antigen
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • Octamer Transcription Factor-3
  • Pou5f1 protein, mouse
  • RNA, Messenger