Abstract
A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Bacterial / genetics
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Antibody Diversity
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Base Sequence
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Binding Sites, Antibody
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Capsid / genetics*
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Cloning, Molecular
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Gene Expression
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Gene Library*
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Genetic Vectors
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Immunoglobulin Fab Fragments / genetics*
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Molecular Sequence Data
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Oligonucleotides / chemistry
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Recombinant Fusion Proteins
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Tetanus Toxoid / immunology
Substances
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Antibodies, Bacterial
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Immunoglobulin Fab Fragments
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Oligonucleotides
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Recombinant Fusion Proteins
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Tetanus Toxoid