A water-soluble carbon monoxide-releasing molecule (CORM-3) lowers intraocular pressure in rabbits

Br J Ophthalmol. 2009 Feb;93(2):254-7. doi: 10.1136/bjo.2007.137034. Epub 2008 Oct 31.

Abstract

Background: Carbon monoxide-releasing molecules (CORMs) are a novel group of substances that are capable of modulating physiological functions via the liberation of CO.

Aims: This study was undertaken to investigate the effects of CORM-3, a water-soluble CO-releasing agent, on two rabbit models of ocular hypertension.

Methods: Ocular hypertension was induced by injecting alpha-chymotrypsin in the rabbit eye. The dose-response effect of CORM-3 on IOP was assessed by topical administration of the drug (0.001, 0.01, 0.1 and 1%). Ocular hypertension was also obtained by weekly subconjunctival injection of betamethasone, and animals were treated topically with CORM-3. A group of animals in both models was treated with the inactive form of the drug (iCORM-3).

Results: CORM-3 induced a dose-dependent reduction in IOP in rabbits treated with alpha-chymotrypsin. A similar reduction in IOP was observed in rabbits with betamethasone-induced ocular hypertension treated with the drug. Treatment with the iCORM-3 had no effect on IOP in both models.

Conclusions: Treatment with CORM-3 is associated with a reduction in IOP in two different rabbit models of ocular hypertension. These results support previous findings on the effect of haem oxygenase-derived CO on IOP and suggest a direct involvement of CO system in the regulation of ocular pressure probably through the modulation of aqueous humour dynamics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antihypertensive Agents / administration & dosage
  • Antihypertensive Agents / therapeutic use*
  • Chymotrypsin
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Drug Evaluation, Preclinical / methods
  • Intraocular Pressure / drug effects
  • Male
  • Ocular Hypertension / chemically induced
  • Ocular Hypertension / drug therapy*
  • Ocular Hypertension / physiopathology
  • Organometallic Compounds / administration & dosage
  • Organometallic Compounds / therapeutic use*
  • Rabbits

Substances

  • Antihypertensive Agents
  • Organometallic Compounds
  • tricarbonylchloro(glycinato)ruthenium(II)
  • Chymotrypsin
  • alpha-chymotrypsin