Epstein-Barr virus renders the infected natural killer cell line, NKL resistant to doxorubicin-induced apoptosis

Y Isobe; K Sugimoto; I Matsuura; K Takada; K Oshimi

doi:10.1038/sj.bjc.6604764

Epstein-Barr virus renders the infected natural killer cell line, NKL resistant to doxorubicin-induced apoptosis

Br J Cancer. 2008 Dec 2;99(11):1816-22. doi: 10.1038/sj.bjc.6604764. Epub 2008 Nov 4.

Authors

Y Isobe¹, K Sugimoto, I Matsuura, K Takada, K Oshimi

Affiliation

¹ Department of Hematology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan.

Abstract

We established two Epstein-Barr virus (EBV)-infected NKL sublines, which acquired stress resistant phenotype against DNA damage and starvation compared with EBV-negative NKL. EBV-rendered doxorubicin resistance at least partially through NF-kappaB activation and the resultant sustenance of antiapoptotic proteins including Bcl-X(L) and FLIP(L/S).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis / drug effects*
Apoptosis / physiology
CASP8 and FADD-Like Apoptosis Regulating Protein / metabolism
Cell Line, Tumor
Doxorubicin / pharmacology*
Drug Resistance, Neoplasm / physiology*
Epstein-Barr Virus Infections / genetics
Epstein-Barr Virus Infections / metabolism*
Flow Cytometry
Herpesvirus 4, Human
Humans
Immunoblotting
Killer Cells, Natural / metabolism
Killer Cells, Natural / virology*
NF-kappa B / metabolism
bcl-X Protein / metabolism

Substances

CASP8 and FADD-Like Apoptosis Regulating Protein
NF-kappa B
bcl-X Protein
Doxorubicin