Lack of functional alpha-lactalbumin prevents involution in Cape fur seals and identifies the protein as an apoptotic milk factor in mammary gland involution

BMC Biol. 2008 Nov 6:6:48. doi: 10.1186/1741-7007-6-48.

Abstract

Background: The mammary gland undergoes a sophisticated programme of developmental changes during pregnancy/lactation. However, little is known about processes involving initiation of apoptosis at involution following weaning. We used fur seals as models to study the molecular process of involution as these animals display a unique mammary gland phenotype. Fur seals have long lactation periods whereby mothers cycle between secreting copious quantities of milk for 2 to 3 days suckling pups on land, with trips to sea alone to forage for up to 23 days during which time mammary glands remain active without initiating apoptosis/involution.

Results: We show the molecular basis by which alpha-lactalbumin (LALBA), a secreted milk protein, is absent in Cape fur seals and demonstrate an apoptotic function for LALBA when exposed to mammary cells.

Conclusion: We propose that apoptosis does not occur in fur seal mammary glands due to lack of LALBA in fur seal milk, allowing evasion of involution during a foraging trip. Our work identifies LALBA as a milk factor that feeds back on the mammary gland to regulate involution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis*
  • Cell Line
  • Cells, Cultured
  • Female
  • Fur Seals / physiology*
  • Gene Expression Regulation
  • Humans
  • Lactalbumin / genetics
  • Lactalbumin / metabolism*
  • Mammary Glands, Animal / chemistry*
  • Mammary Glands, Animal / cytology*
  • Mammary Glands, Animal / metabolism
  • Mice
  • Mutation
  • Pregnancy
  • Promoter Regions, Genetic
  • Protein Biosynthesis
  • RNA Precursors / analysis
  • RNA, Messenger / analysis
  • Time Factors

Substances

  • RNA Precursors
  • RNA, Messenger
  • Lactalbumin