X-ray structure of the metal-sensor CnrX in both the apo- and copper-bound forms

FEBS Lett. 2008 Nov 26;582(28):3954-8. doi: 10.1016/j.febslet.2008.10.042. Epub 2008 Nov 4.

Abstract

Both the X-ray structures of the apo- and the copper-bound forms of the metal-sensor domain (residues 31-148) of CnrX from Cupriavidus metallidurans CH34 were obtained at 1.74A resolution from a selenomethionine derivative. This four-helix hooked-hairpin is the first structure of a metal-sensor in an ECF-type signaling pathway. The copper ion is bound in a type 2-like center with a 3N1O coordination in the equatorial plane and shows an unprecedented remote fifth axial ligand with Met93 contributing a weak S-Cu bond. The signal onset cannot be explained by conformational changes associated with CnrX metallation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Apoproteins / chemistry*
  • Apoproteins / metabolism
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism
  • Copper / chemistry*
  • Copper / metabolism
  • Crystallography, X-Ray
  • Cupriavidus / metabolism*
  • Dimerization
  • Molecular Sequence Data
  • Periplasmic Proteins / chemistry*
  • Periplasmic Proteins / metabolism
  • Protein Conformation
  • Protein Structure, Tertiary

Substances

  • Apoproteins
  • Bacterial Proteins
  • Periplasmic Proteins
  • Copper