Difference in number of loci of swine leukocyte antigen classical class I genes among haplotypes

Genomics. 2009 Mar;93(3):261-73. doi: 10.1016/j.ygeno.2008.10.004. Epub 2008 Dec 3.

Abstract

The structure of the entire genomic region of swine leukocyte antigen (SLA)-the porcine major histocompatibility complex--was recently elucidated in a particular haplotype named Hp-1.0 (H01). However, it has been suggested that there are differences in the number of loci of SLA genes, particularly classical class I genes, among haplotypes. To clarify the between-haplotype copy number variance in genes of the SLA region, we sequenced the genomic region carrying SLA classical class I genes on two different haplotypes, revealing increments of up to six in the number of classical class I genes in a single haplotype. All of the SLA-1(-like) (SLA-1 and newly designated SLA-12) and SLA-3 genes detected in the haplotypes thus analyzed were transcribed in the individual. The process by which duplication of SLA classical class I genes was likely to have occurred was interpreted from an analysis of repetitive sequences adjacent to the duplicated class I genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromosomes, Artificial, Bacterial
  • Gene Duplication
  • Gene Expression Regulation
  • Genes, MHC Class I / genetics*
  • Genetic Variation*
  • Haplotypes
  • Histocompatibility Antigens Class I / chemistry
  • Histocompatibility Antigens Class I / genetics*
  • Histocompatibility Antigens Class I / metabolism
  • Histocompatibility Antigens Class II
  • Molecular Sequence Data
  • Phylogeny
  • Swine / genetics*
  • Swine / immunology

Substances

  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • swine leukocyte antigen

Associated data

  • GENBANK/AP009553
  • GENBANK/AP009554
  • GENBANK/AP009555
  • GENBANK/AP009556
  • GENBANK/AP009557
  • GENBANK/AP009558
  • GENBANK/AP009559