Abstract
Three nitrogen mustard-sensitive lines of Chinese hamster ovary cells were isolated from mutagenized cultures using the procedure of Thompson et al. (1980). The lines, designated NM1, NM2 and NM3, were 2.1-, 17- and 6.8-fold more sensitive to nitrogen mustard, respectively, than their parent, wild-type, line as determined by the dose required to kill 90% of the cells, IC90. Patterns of cross-sensitivity to other DNA-damaging agents including ultraviolet light, cis-diamminedichloroplatinum, and other alkylating agents were determined for each line. Analysis of these results suggests that the phenotypes of the mutant lines are different from those lines reported previously.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aminacrine / analogs & derivatives
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Aminacrine / pharmacology
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Animals
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Cells, Cultured*
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Cisplatin / pharmacology
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Cricetinae
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Cross Reactions
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DNA / drug effects*
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DNA / radiation effects
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Dose-Response Relationship, Drug
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Female
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Gamma Rays / adverse effects
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In Vitro Techniques
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Mechlorethamine / pharmacology*
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Melphalan / pharmacology
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Mitomycin
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Mitomycins / pharmacology
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Mutagens / pharmacology
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Mutation*
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Nitrogen Mustard Compounds / pharmacology
Substances
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Mitomycins
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Mutagens
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Nitrogen Mustard Compounds
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Mechlorethamine
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Mitomycin
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Aminacrine
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DNA
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acridine half-mustard
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Cisplatin
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Melphalan