A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples

Claus Bo Svendsen; Mette Boye; Carsten Struve; Karen A Krogfelt

doi:10.1016/j.mimet.2008.10.012

A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples

J Microbiol Methods. 2009 Mar;76(3):301-4. doi: 10.1016/j.mimet.2008.10.012. Epub 2008 Nov 5.

Authors

Claus Bo Svendsen¹, Mette Boye, Carsten Struve, Karen A Krogfelt

Affiliation

¹ Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark. [email protected] <[email protected]>

PMID: 19007824
DOI: 10.1016/j.mimet.2008.10.012

Abstract

A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Rickettsia was found without problems of cross-reactions with bacterial species shown to cross-react serologically.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques
In Situ Hybridization, Fluorescence / methods*
Oligonucleotide Probes
RNA, Bacterial / analysis
RNA, Ribosomal, 16S / analysis
Rickettsia / isolation & purification*
Sensitivity and Specificity
Species Specificity

Substances

Oligonucleotide Probes
RNA, Bacterial
RNA, Ribosomal, 16S