Abstract
Myeloid dendritic cells (mDC) activated with a B7-DC-specific cross-linking IgM Ab (B7-DC XAb) take up and retain Ag and interact with T cell compartments to affect a number of biologic changes that together cause strong antitumor responses and blockade of inflammatory airway disease in animal models. The molecular events mediating the initial responses in mDC remain unclear. In this study we show that B7-DC XAb caused rapid phosphorylation of the adaptor protein DAP12 and intracellular kinases Syk and phospholipase C-gamma1. Pretreatment of mDC with the Syk inhibitor piceatannol blocked B7-DC XAb-induced Ag uptake with a concomitant loss of tumor protection in mice. Vaccination with tumor lysate-pulsed wild-type B7-DC XAb-activated mDC, but not TREM-2 knockout XAb-activated mDC, protected mice from lethal melanoma challenge. Multimolecular caps appeared within minutes of B7-DC XAb binding to either human or mouse mDC, and FRET analysis showed that class II, CD80, CD86, and TREM-2 are recruited in tight association on the cell surface. When TREM-2 expression was reduced in wild-type mDC using short hairpin RNA or by using mDC from TREM-2 knockout mice, in vitro DC failed to take up Ag after B7-DC XAb stimulation. These results directly link TREM-2 signaling with one change in the mDC phenotype that occurs in response to this unique Ab. The parallel signaling events observed in both human and mouse mDC support the hypothesis that B7-DC cross-linking may be useful as a therapeutic immune modulator in human patients.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Retracted Publication
MeSH terms
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Adaptor Proteins, Signal Transducing / genetics
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Adaptor Proteins, Signal Transducing / immunology
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Animals
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Antibodies / pharmacology
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Antigens / immunology*
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B7-1 Antigen / genetics
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B7-1 Antigen / immunology
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B7-2 Antigen / genetics
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B7-2 Antigen / immunology
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Dendritic Cells / immunology*
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Gene Expression Regulation / drug effects
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Gene Expression Regulation / genetics
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Gene Expression Regulation / immunology
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Histocompatibility Antigens Class II / genetics
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Histocompatibility Antigens Class II / immunology
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Humans
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Immunologic Capping / drug effects
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Immunologic Capping / genetics
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Immunologic Capping / immunology
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Inflammation / genetics
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Inflammation / immunology
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Intracellular Signaling Peptides and Proteins / antagonists & inhibitors
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / immunology
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Melanoma / genetics
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Melanoma / immunology
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Membrane Glycoproteins / antagonists & inhibitors
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology*
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Membrane Proteins / genetics
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Membrane Proteins / immunology
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Mice
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Mice, Knockout
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Myeloid Cells / immunology*
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Neoplasms, Experimental / genetics
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Neoplasms, Experimental / immunology
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Phospholipase C gamma / genetics
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Phospholipase C gamma / immunology
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Phosphorylation / drug effects
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Phosphorylation / genetics
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Phosphorylation / immunology
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Protein-Tyrosine Kinases / antagonists & inhibitors
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Protein-Tyrosine Kinases / genetics
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Protein-Tyrosine Kinases / immunology
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Receptors, Immunologic / antagonists & inhibitors
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Receptors, Immunologic / genetics
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Receptors, Immunologic / immunology*
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Respiration Disorders / genetics
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Respiration Disorders / immunology
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Signal Transduction / drug effects
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Signal Transduction / genetics
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Signal Transduction / immunology*
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Stilbenes / pharmacology
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Syk Kinase
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T-Lymphocytes / immunology
Substances
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Adaptor Proteins, Signal Transducing
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Antibodies
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Antigens
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B7-1 Antigen
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B7-2 Antigen
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CD86 protein, human
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Cd86 protein, mouse
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Histocompatibility Antigens Class II
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Intracellular Signaling Peptides and Proteins
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Membrane Glycoproteins
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Membrane Proteins
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Receptors, Immunologic
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Stilbenes
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TREM2 protein, human
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TYROBP protein, human
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Trem2 protein, mouse
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Tyrobp protein, mouse
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3,3',4,5'-tetrahydroxystilbene
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Protein-Tyrosine Kinases
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SYK protein, human
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Syk Kinase
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Syk protein, mouse
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Phospholipase C gamma