[RNA interference of hnRNP B1 gene in human A549 lung cells]

Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Sep;39(5):805-7.
[Article in Chinese]

Abstract

Objective: To test the effect of RNA interference of hnRNP Bx gene in human lung cell line A549.

Methods: The RNAs of cell line A549 were interfered by the RNA interference technique. The hnRNP B1 mRNAs were detected by fluorescence quantity RT-PCR. The proteins of hnRNP B1 were detected by Western blot technique. The growth rate of the cells was analysed by MTT.

Results: The growth of the cells was suppressed significantly. The mRNA and protein expressions of hnRNP B1 were inhibited.

Conclusion: The expression of hnRNP B1 can be inhibited by RNA interference. RNA interference could become a possible clinical therapy for lung cancers.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Heterogeneous-Nuclear Ribonucleoprotein Group A-B / biosynthesis
  • Heterogeneous-Nuclear Ribonucleoprotein Group A-B / genetics*
  • Humans
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology
  • RNA Interference
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • RNA, Small Interfering / genetics*
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Heterogeneous-Nuclear Ribonucleoprotein Group A-B
  • RNA, Messenger
  • RNA, Small Interfering
  • hnRNP A2