[BFGF entraped in cationic liposomes as adjuvant: optimization of technical parameters]

Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Sep;39(5):839-41, 846.
[Article in Chinese]

Abstract

Objective: To test the immune efficiency of bFGF entraped in cationic liposomes as adjuvant in vivo.

Methods: The technical parameters on encapsulation were tested in each step to gain high encapsulation efficiencies, which included lipid composition, weight ratio of protein and lipids, liposome extrusion, and different conditions of freeze-thawing. The bFGF in cationic liposome, Freund's adjuvant, or PBS were injected (four times) to the four-week old Balb/c mice to test the immune responses. The serum antibody was measured by ELISA 13 days after each injection.

Results: Maximal encapsulation efficiency (about 50%) was achieved through optimized technical parameters. Cationic liposome demonstrated satisfied immune efficiency as adjuvant.

Conclusion: Cationic liposome is a safe and effective immunological adjuvant.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Cations / chemistry
  • Cations / immunology
  • Drug Carriers
  • Fibroblast Growth Factor 2 / administration & dosage
  • Fibroblast Growth Factor 2 / immunology*
  • Freund's Adjuvant / immunology
  • Immunization*
  • Liposomes / chemistry
  • Liposomes / immunology*
  • Mice
  • Mice, Inbred BALB C

Substances

  • Cations
  • Drug Carriers
  • Liposomes
  • Fibroblast Growth Factor 2
  • Freund's Adjuvant