On-chip tryptic digest with direct coupling to ESI-MS using magnetic particles

Electrophoresis. 2008 Dec;29(24):4944-7. doi: 10.1002/elps.200800431.

Abstract

As a step toward a fully automated front-end microfluidic chip for MS proteomics, we propose a system capable of performing online tryptic digest and ESI-MS, using a replaceable on-chip digestion microcolumn based on self-assembled magnetic particles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dimethylpolysiloxanes / chemistry
  • Magnetics
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods
  • Proteins / analysis
  • Proteomics / methods
  • Spectrometry, Mass, Electrospray Ionization / instrumentation*
  • Spectrometry, Mass, Electrospray Ionization / methods
  • Trypsin / chemistry*

Substances

  • Dimethylpolysiloxanes
  • Proteins
  • baysilon
  • Trypsin