Abstract
The CP23 gene of Cryptosporidium parvum strain isolated from Changchun in China was expressed in Escherichia coli BL21 strain and was purified as a recombinant protein. An indirect ELISA assay (CP23-ELISA) for antibody detection was established using the purified recombinant CP23 protein. Antigen coating conditions and serum dilution for the CP23-ELISA were optimized. The S/P ratio of the absorbency value was calculated in the CP23-ELISA to evaluate the serum antibody level of the field cow samples. It indicated that the CP23-ELISA assay, which was more convenient and easier to prepare than traditional methods, was a good candidate for evaluation of C. parvum exposure to domestic animal in field.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Helminth / blood*
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Antibodies, Helminth / immunology
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Antigens, Protozoan* / immunology
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Antigens, Protozoan* / isolation & purification
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Blotting, Western / veterinary
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Cattle
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Cattle Diseases / diagnosis*
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Cattle Diseases / immunology
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Cattle Diseases / parasitology
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Cryptosporidiosis / diagnosis
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Cryptosporidiosis / parasitology
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Cryptosporidiosis / veterinary*
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Cryptosporidium parvum / immunology*
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Cryptosporidium parvum / isolation & purification
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Electrophoresis, Polyacrylamide Gel / veterinary
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Enzyme-Linked Immunosorbent Assay / methods
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Enzyme-Linked Immunosorbent Assay / standards
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Enzyme-Linked Immunosorbent Assay / veterinary*
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Feces / parasitology
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Mice
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Mice, Inbred BALB C
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Recombinant Proteins / immunology
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Recombinant Proteins / isolation & purification
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Sensitivity and Specificity
Substances
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Antibodies, Helminth
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Antigens, Protozoan
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Recombinant Proteins