A new bio-active surface for protein immobilisation via copper-free 'click' between azido SAM and alkynyl Fischer carbene complex

Chem Commun (Camb). 2008 Dec 7:(45):5957-9. doi: 10.1039/b813296h. Epub 2008 Oct 9.

Abstract

Fischer carbene complex anchored on glass or silicon surface using a Cu-free 'click' reaction allows facile and swift covalent grafting of protein molecules like Bovine Serum Albumin (BSA).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Azides / chemical synthesis*
  • Azides / chemistry
  • Cattle
  • Dansyl Compounds / chemical synthesis
  • Dansyl Compounds / chemistry
  • Immobilized Proteins / chemistry*
  • Methane / analogs & derivatives*
  • Methane / chemistry
  • Serum Albumin, Bovine / chemistry
  • Silicates / chemical synthesis
  • Silicates / chemistry
  • Spectrometry, Fluorescence

Substances

  • Azides
  • Dansyl Compounds
  • Immobilized Proteins
  • Silicates
  • carbene
  • Serum Albumin, Bovine
  • Methane
  • dansyl chloride