Abstract
The binding affinity of (S)-2-amino-6-nitrohexanoic acid to human arginase I was studied using surface plasmon resonance (K(d) = 60 microM), and the X-ray crystal structure of the enzyme-inhibitor complex was determined at 1.6 A resolution to reveal multiple nitro-metal coordination interactions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminocaproates
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Arginase / antagonists & inhibitors
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Arginase / chemistry*
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Arginine / chemistry
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Biophysical Phenomena
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Caproates / chemistry*
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Caproates / pharmacology
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Chemistry, Organic / methods
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Crystallography, X-Ray / methods
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Humans
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Kinetics
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Ligands
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Manganese / chemistry*
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Metals / chemistry*
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Models, Chemical
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Molecular Structure
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Nitro Compounds / chemistry*
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Nitro Compounds / pharmacology
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Nitrogen / chemistry*
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Protein Binding
Substances
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(S)-2-amino-6-nitrohexanoic acid
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Aminocaproates
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Caproates
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Ligands
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Metals
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Nitro Compounds
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hexanoic acid
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Manganese
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Arginine
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Arginase
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Nitrogen