Abstract
Exposure of hamster amelanotic melanoma cells to L-dihydroxyphenylalanine (L-DOPA) resulted in a time dependent increase of cell pigmentation, tyrosinase concentration and activity with peak after 24 hours. Northern blot analysis showed a small but reproducible increase of tyrosinase mRNA after 3 hours and a decrease below the control level after 9 hours. After 24 and 48 hours tyrosinase mRNA was undetectable. It is suggested that L-DOPA or its oxidation products can stimulate intracellular tyrosinase concentration and regulate tyrosinase mRNA level both in positive and negative fashion.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cloning, Molecular
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Cricetinae
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Drug Administration Schedule
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Gene Expression Regulation, Enzymologic / drug effects*
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Gene Expression Regulation, Neoplastic / drug effects
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Intracellular Fluid / metabolism
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Levodopa / pharmacology*
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Melanins / biosynthesis
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Melanoma / enzymology*
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Melanoma / genetics
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Melanoma / pathology
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Monophenol Monooxygenase / genetics
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Monophenol Monooxygenase / metabolism*
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Protein Biosynthesis
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Tumor Cells, Cultured
Substances
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Melanins
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RNA, Messenger
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Levodopa
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Monophenol Monooxygenase