Assessment of the feasibility of exon 45-55 multiexon skipping for Duchenne muscular dystrophy

BMC Med Genet. 2008 Dec 1:9:105. doi: 10.1186/1471-2350-9-105.

Abstract

Background: The specific skipping of an exon, induced by antisense oligonucleotides (AON) during splicing, has shown to be a promising therapeutic approach for Duchenne muscular dystrophy (DMD) patients. As different mutations require skipping of different exons, this approach is mutation dependent. The skipping of an entire stretch of exons (e.g. exons 45 to 55) has recently been suggested as an approach applicable to larger groups of patients. However, this multiexon skipping approach is technically challenging. The levels of intended multiexon skips are typically low and highly variable, and may be dependent on the order of intron removal. We hypothesized that the splicing order might favor the induction of multiexon 45-55 skipping.

Methods: We here tested the feasibility of inducing multiexon 45-55 in control and patient muscle cell cultures using various AON cocktails.

Results: In all experiments, the exon 45-55 skip frequencies were minimal and comparable to those observed in untreated cells.

Conclusion: We conclude that current state of the art does not sufficiently support clinical development of multiexon skipping for DMD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Cell Differentiation
  • Cells, Cultured
  • Exons*
  • Genetic Therapy
  • Humans
  • Muscle Fibers, Skeletal / pathology
  • Muscular Dystrophy, Duchenne / genetics*
  • Muscular Dystrophy, Duchenne / pathology
  • Muscular Dystrophy, Duchenne / therapy
  • Myoblasts, Skeletal / pathology
  • Oligonucleotides, Antisense / genetics*
  • Open Reading Frames
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Deletion

Substances

  • Oligonucleotides, Antisense