Crystallization and preliminary X-ray analysis of the human androgen receptor ligand-binding domain with a coactivator-like peptide and selective androgen receptor modulators

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Dec 1;64(Pt 12):1159-62. doi: 10.1107/S1744309108036683. Epub 2008 Nov 28.

Abstract

The ligand-binding domain of the human androgen receptor has been cloned, overproduced and crystallized in the presence of a coactivator-like 11-mer peptide and two different nonsteroidal ligands. The crystals of the two ternary complexes were isomorphous and belonged to space group P2(1)2(1)2(1), with one molecule in the asymmetric unit. They diffracted to 1.7 and 1.95 A resolution, respectively. Structure determination of these two complexes will help in understanding the mode of binding of selective nonsteroidal androgens versus endogenous steroidal ligands and possibly the origin of their tissue selectivity.

MeSH terms

  • Binding Sites
  • Crystallization
  • Crystallography, X-Ray
  • Humans
  • Ligands
  • Peptides / chemistry
  • Peptides / metabolism*
  • Protein Structure, Tertiary
  • Receptors, Androgen / chemistry*
  • Receptors, Androgen / isolation & purification
  • Receptors, Androgen / metabolism

Substances

  • AR protein, human
  • Ligands
  • Peptides
  • Receptors, Androgen