Abstract
The spoIIA operon encodes three genes, including the structural gene for a sporulation-induced sigma factor sigma F. We used deletion analysis of spoIIA-lacZ fusions to define the location of the spoIIA promoter. We found that sigma H-RNA polymerase transcribes spoIIA accurately in vitro and propose that sigma H directs transcription of spoIIA during sporulation.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacillus subtilis / genetics*
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Bacillus subtilis / physiology
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Bacterial Proteins / genetics*
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Base Sequence
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Chromosome Deletion
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Cloning, Molecular
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Molecular Sequence Data
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Promoter Regions, Genetic / physiology
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Sigma Factor / physiology
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Spores, Bacterial
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Transcription Factors*
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Transcription, Genetic*
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beta-Galactosidase / biosynthesis
Substances
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Bacterial Proteins
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Sigma Factor
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Transcription Factors
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spoIIR protein, Bacillus subtilis
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spore-specific proteins, Bacillus
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beta-Galactosidase
Associated data
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GENBANK/M17643
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GENBANK/M62974
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GENBANK/M62975
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GENBANK/M74306
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GENBANK/S70115
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GENBANK/S70117
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GENBANK/S70121
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GENBANK/S70125
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GENBANK/S70128
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GENBANK/S70130