An SfiI restriction map of the Bacillus subtilis 168 genome

M Amjad; J M Castro; H Sandoval; J J Wu; M Yang; D J Henner; P J Piggot

doi:10.1016/0378-1119(91)90219-2

An SfiI restriction map of the Bacillus subtilis 168 genome

Gene. 1991 May 15;101(1):15-21. doi: 10.1016/0378-1119(91)90219-2.

Authors

M Amjad¹, J M Castro, H Sandoval, J J Wu, M Yang, D J Henner, P J Piggot

Affiliation

¹ Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140.

PMID: 1905666
DOI: 10.1016/0378-1119(91)90219-2

Abstract

A restriction map of 24 SfiI (GGCCN4/NGGCC) restriction fragments has been constructed for the Bacillus subtilis genome. The combined sizes of the fragments indicate a genome size of approx. 4.2 Mb. The SfiI fragments range in size from 7-730 kb. Genetic markers have been located on 19 of the SfiI fragments, and 69 genetic markers have been assigned to the SfiI restriction map.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacillus subtilis / genetics*
Blotting, Southern
Deoxyribonucleases, Type II Site-Specific*
Electrophoresis
Genes, Bacterial*
Genetic Markers
Restriction Mapping*

Substances

Genetic Markers
endodeoxyribonuclease SfiI
Deoxyribonucleases, Type II Site-Specific
GCGGCCGC-specific type II deoxyribonucleases