Changes in regulatory microRNA expression in myocardium of heart failure patients on left ventricular assist device support

J Heart Lung Transplant. 2008 Dec;27(12):1282-5. doi: 10.1016/j.healun.2008.09.005.

Abstract

Background: Left ventricular assist device support in heart failure patients leads to changes in mRNA and protein expression in the myocardium.

Methods: MicroRNA's are important regulators of various cellular processes, so changes in their expression were tested by Q-PCR methods.

Results: LVAD support (independently of the duration) leads to a decrease of the expression of miR-1, miR-133a and miR-133b in DCM patients but to an increase in expression in IHD patients. The expression of miR's pre- and post-LVAD in heart failure patients was low compared to the level of miR's in control myocardial tissue.

Conclusions: Alterations in miRs expression might also be important during repair processes in IHD patients.

MeSH terms

  • Adult
  • Autopsy
  • Female
  • Heart / physiopathology*
  • Heart Failure / genetics*
  • Heart Failure / surgery*
  • Heart Transplantation*
  • Heart-Assist Devices*
  • Humans
  • Male
  • MicroRNAs / genetics*
  • MicroRNAs / isolation & purification
  • Middle Aged
  • Myocardium / pathology
  • RNA / genetics
  • RNA / isolation & purification
  • Reference Values
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tissue Donors
  • Ventricular Dysfunction, Left / genetics
  • Ventricular Dysfunction, Left / physiopathology
  • Ventricular Dysfunction, Left / surgery

Substances

  • MicroRNAs
  • RNA