Abstract
We have established a cell line derived from a pleural effusion of breast scirrhous carcinoma. This cell line presented immunohistochemically negative for estrogen receptor and slightly positive for progesterone receptor, and positive for c-erbB/HER2/neu. However the original tumor was found to be positive for estrogen receptor and progesterone receptor and slightly positive for c-erbB/HER2/neu expression. Enzyme and electrochemiluminescence immunoassays of tumor markers in the conditioned medium by the cell line revealed they secrete CA15-3, NCC-ST-439 and HER2 protein. We believe the cell line will contribute to the therapeutic study of malignant breast cancer.
MeSH terms
-
Adenocarcinoma, Scirrhous* / genetics
-
Adenocarcinoma, Scirrhous* / pathology
-
Adenocarcinoma, Scirrhous* / ultrastructure
-
Animals
-
Antigens, Tumor-Associated, Carbohydrate / analysis
-
Biomarkers, Tumor / analysis*
-
Breast Neoplasms* / genetics
-
Breast Neoplasms* / pathology
-
Breast Neoplasms* / ultrastructure
-
Cell Line, Tumor
-
Female
-
Gene Expression Regulation, Neoplastic
-
Humans
-
Karyotyping
-
Mice
-
Middle Aged
-
Mucin-1 / analysis
-
Neoplasm Transplantation
-
Pleural Effusion, Malignant* / cytology
-
Pleural Effusion, Malignant* / genetics
-
Pleural Effusion, Malignant* / pathology
-
Receptor, ErbB-2* / analysis
-
Receptors, Estrogen
-
Receptors, Progesterone
Substances
-
Antigens, Tumor-Associated, Carbohydrate
-
Biomarkers, Tumor
-
Mucin-1
-
Receptors, Estrogen
-
Receptors, Progesterone
-
ST 439 antigen, human
-
Receptor, ErbB-2