Sensitive liquid chromatography-electrospray mass spectrometry allows for the analysis of the O-glycosylation of immunoprecipitated proteins from cells or tissues: application to MUC1 glycosylation in cancer

Malin Bäckström; Kristina A Thomsson; Hasse Karlsson; Gunnar C Hansson

doi:10.1021/pr800713h

Sensitive liquid chromatography-electrospray mass spectrometry allows for the analysis of the O-glycosylation of immunoprecipitated proteins from cells or tissues: application to MUC1 glycosylation in cancer

J Proteome Res. 2009 Feb;8(2):538-45. doi: 10.1021/pr800713h.

Authors

Malin Bäckström¹, Kristina A Thomsson, Hasse Karlsson, Gunnar C Hansson

Affiliation

¹ Institute of Biomedicine, Department of Medical Biochemistry, University of Gothenburg, Sweden. [email protected]

PMID: 19072658
DOI: 10.1021/pr800713h

Abstract

We have analyzed the structures of the glycans on immunoprecipitated proteins from small amounts of cell or tissue lysates, by liquid-chromatography electrospray mass spectrometry (LC-ESI-MS) and MS/MS. The sensitive and specific method was applied to the analysis of the O-glycosylation of MUC1 in breast, prostate and gastric cancer, including analysis of a patient tumor specimen. The method will be applicable for the glycosylation analysis of individual proteins.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Conformation
Carbohydrate Sequence
Cell Line
Chromatography, Liquid / methods*
Glycosylation
Humans
Immunoprecipitation
Molecular Sequence Data
Mucin-1* / chemistry
Mucin-1* / metabolism
Neoplasms* / chemistry
Neoplasms* / diagnosis
Neoplasms* / metabolism
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization / methods*

Substances

MUC1 protein, human
Mucin-1