Abstract
An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-acetyl-beta-glucosaminidase, yielding products which could be analysed by 1H-NMR spectroscopy, thereby giving conclusive data about the location of the extra units in the intact structures.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylglucosaminidase / metabolism
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Amino Sugars / chemistry*
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Amino Sugars / metabolism
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Animals
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Carbohydrate Sequence
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Cell Line
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Cricetinae
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Erythropoietin / chemistry
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Glycoside Hydrolases*
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Humans
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Magnetic Resonance Spectroscopy
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Molecular Sequence Data
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Oligosaccharides / chemistry*
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Oligosaccharides / metabolism
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beta-Galactosidase / metabolism
Substances
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Amino Sugars
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Oligosaccharides
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sialooligosaccharides
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Erythropoietin
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N-acetyllactosamine
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Glycoside Hydrolases
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keratan-sulfate endo-1,4-beta-galactosidase
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beta-Galactosidase
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Acetylglucosaminidase