Hemolytic activity of Trichomonas gallinae isolates does not correspond with clinical virulence

Vet Parasitol. 2009 Mar 23;160(3-4):221-4. doi: 10.1016/j.vetpar.2008.10.098. Epub 2008 Nov 6.

Abstract

The hemolytic activity of 22 Trichomonas gallinae isolates was investigated using an 18h erythrocyte hemolysis assay which has been shown to correlate with the clinical virulence of T. vaginalis. Absorbance of the assay supernatants was measured at 540nm and expressed as percentage of complete hemolysis. Mean hemolytic activity of the T. gallinae isolates ranged from 3.5% to 53.4% and did not correspond with clinical virulence. The results of this investigation suggest hemolytic activity is not a useful in vitro virulence assay for T. gallinae.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bird Diseases / parasitology
  • Bird Diseases / pathology
  • Birds
  • Chickens / blood
  • Culture Techniques / veterinary*
  • Erythrocytes / parasitology*
  • Hemolysin Proteins / analysis*
  • Hemolysis
  • Poultry Diseases / parasitology
  • Poultry Diseases / pathology
  • Trichomonas / pathogenicity*
  • Trichomonas Infections / parasitology
  • Trichomonas Infections / pathology
  • Trichomonas Infections / veterinary*
  • Virulence

Substances

  • Hemolysin Proteins