Fetal surgery of extremity amniotic bands: an experimental model of in utero limb salvage in fetal lamb

J Pediatr Orthop. 2009 Jan-Feb;29(1):98-102. doi: 10.1097/BPO.0b013e318192196e.

Abstract

Background: Extremity amniotic band (EAB) syndrome can cause an intrauterine amputation as a result of a mechanical effect with progressive strangulation. The aim of the study is to assess the use of fetal surgery of EABs with risk of amputation in the ovine fetus.

Methods: Right limbs of fifteen 60-day-gestational age fetal sheep were ligated with silk suture at the infracondylar level. Left limbs were used for paired comparison. Fetuses were randomized into 3 groups: an early-repair group (n = 5), a late-repair group (n = 5), and a nonrepaired group (n = 5). The limbs of the 2 repaired groups underwent fetal release. The limbs obtained from at-term fetuses were analyzed morphologically, functionally, and radiologically. Statistical analysis with paired test was used to compare data.

Results: Nonrepaired limbs showed amputation or quasi amputation; the repaired ones did not. However, those late-repaired had significantly reduced passive ankle range of motion, shorter limb length, and mild residual changes.

Conclusions: Intrauterine release of potentially severe EABs prevents limb amputation and leads to morphofunctional recovery. Early release shows better results.

Clinical relevance: : Intrauterine amputations caused by EABs in the human fetus might be avoidable by fetal surgery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amniotic Band Syndrome / surgery*
  • Amputation, Traumatic / prevention & control*
  • Animals
  • Ankle Joint / pathology
  • Ankle Joint / surgery
  • Data Interpretation, Statistical
  • Disease Models, Animal
  • Extremities / embryology
  • Extremities / pathology
  • Extremities / surgery*
  • Female
  • Fetus
  • Humans
  • Infant, Newborn
  • Leg Length Inequality / etiology
  • Limb Salvage / methods*
  • Pregnancy
  • Random Allocation
  • Range of Motion, Articular
  • Sheep
  • Time Factors
  • Treatment Outcome