Genotypic characterization of CRF01_AE env genes derived from human immunodeficiency virus type 1-infected patients residing in central Thailand

AIDS Res Hum Retroviruses. 2009 Feb;25(2):229-36. doi: 10.1089/aid.2008.0232.

Abstract

CRF01_AE is a major subtype of human immunodeficiency virus type 1 (HIV-1) circulating in Southeast Asia, including Thailand. HIV-1 env genes were amplified by polymerase chain reaction from blood samples of HIV-1-infected patients residing in Thailand in 2006, and cloned into the pNL4-3-derived reporter viral construct. Generated envelope protein (Env)-recombinant virus was examined for its infectivity, and then 35 infectious CRF01_AE Env-recombinant viruses were selected. Sequencing analysis revealed that the interclone variation of the deduced amino acid sequences was higher in CRF01_AE env genes isolated in 2006 than in those isolated in the early 1990s, suggesting that env gene variation has been increasing gradually among CRF01_AE viruses prevalent in Thailand. We also examined the characteristics of the deduced amino acid sequences of 35 CRF01_AE env genes. Our results may provide useful information to help in better understanding the genotype of env genes of CRF01_AE viruses currently circulating in Thailand.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blood / virology
  • Genotype
  • HIV Infections / virology*
  • HIV-1 / classification
  • HIV-1 / genetics*
  • HIV-1 / isolation & purification*
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction / methods
  • Polymorphism, Genetic*
  • Recombination, Genetic
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Thailand
  • env Gene Products, Human Immunodeficiency Virus / genetics*

Substances

  • env Gene Products, Human Immunodeficiency Virus

Associated data

  • GENBANK/EU743757
  • GENBANK/EU743758
  • GENBANK/EU743759
  • GENBANK/EU743760
  • GENBANK/EU743761
  • GENBANK/EU743762
  • GENBANK/EU743763
  • GENBANK/EU743764
  • GENBANK/EU743765
  • GENBANK/EU743766
  • GENBANK/EU743767
  • GENBANK/EU743768
  • GENBANK/EU743769
  • GENBANK/EU743770
  • GENBANK/EU743771
  • GENBANK/EU743772
  • GENBANK/EU743773
  • GENBANK/EU743774
  • GENBANK/EU743775
  • GENBANK/EU743776
  • GENBANK/EU743777
  • GENBANK/EU743778
  • GENBANK/EU743779
  • GENBANK/EU743780
  • GENBANK/EU743781
  • GENBANK/EU743782
  • GENBANK/EU743783
  • GENBANK/EU743784
  • GENBANK/EU743785
  • GENBANK/EU743786
  • GENBANK/EU743787
  • GENBANK/EU743788
  • GENBANK/EU743789
  • GENBANK/EU743790
  • GENBANK/EU743791
  • GENBANK/EU743792
  • GENBANK/EU743793
  • GENBANK/EU743794