Abstract
Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.
MeSH terms
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Bacterial Toxins / biosynthesis
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Bacterial Toxins / genetics*
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Clostridium Infections / diagnosis*
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Clostridium perfringens / genetics*
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Clostridium perfringens / isolation & purification
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DNA, Bacterial / genetics
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DNA, Ribosomal / genetics
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Fixatives / pharmacology
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Formaldehyde / pharmacology
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Genotype
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Humans
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Paraffin Embedding
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Polymerase Chain Reaction / methods*
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RNA, Ribosomal, 16S / genetics
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Tissue Preservation / methods*
Substances
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Bacterial Toxins
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DNA, Bacterial
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DNA, Ribosomal
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Fixatives
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RNA, Ribosomal, 16S
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Formaldehyde