Carbapenem resistance among Pseudomonas aeruginosa strains from India: evidence for nationwide endemicity of multiple metallo-beta-lactamase clones (VIM-2, -5, -6, and -11 and the newly characterized VIM-18)

Antimicrob Agents Chemother. 2009 Mar;53(3):1225-7. doi: 10.1128/AAC.01011-08. Epub 2008 Dec 29.

Abstract

Among 57 metallo-beta-lactamase (MbetaL)-producing Pseudomonas spp. detected in India during 2006, five bla(VIM) genes were found, including a newly characterized bla(VIM) gene. Pseudomonas aeruginosa strains were clustered in 33 ribotypes with clones found in multiple hospitals. Several types of bla(VIM-2)-carrying integrons were detected. The newly characterized variant VIM-18 showed a 4-amino-acid deletion compared to other VIM variants. In this study, we show that VIM-producing Pseudomonas spp. were highly prevalent in India with a great diversity of bla(VIM) types and MbetaL-carrying integrons.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics
  • Base Sequence
  • Carbapenems / pharmacology*
  • Drug Resistance, Bacterial / genetics*
  • Humans
  • India / epidemiology
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Prevalence
  • Pseudomonas aeruginosa* / drug effects
  • Pseudomonas aeruginosa* / enzymology
  • Pseudomonas aeruginosa* / genetics
  • Pseudomonas aeruginosa* / isolation & purification
  • Ribotyping
  • beta-Lactamases / classification*
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carbapenems
  • beta-lactamase bla(vim-2)
  • beta-Lactamases
  • beta-lactamase VIM-11, Pseudomonas aeruginosa
  • beta-lactamase VIM-18, Pseudomonas aeruginosa
  • beta-lactamase VIM-5, Pseudomonas aeruginosa
  • beta-lactmase VIM-6, Pseudomonas aeruginosa

Associated data

  • GENBANK/AM778091