Abstract
Recent publications have shown that mechanical stress can regulate the direction of stem cell differentiation. The exact mechanobiological effects of pressure on initial osteodifferentiation have not been determined. Here, we show that ERK signaling participates in early osteodifferentiation and plays a positive but non-critical role in mechanotransduction, whereas p38 MAPK is not involved in this process. Moreover, our findings provide evidence that in response to both types of pressure with high sensitivity, Wnt10b mRNA is ERK-dependent whereas Wnt4 mRNA is upregulated by treatment of the inhibitor of ERK signaling. The findings suggest novel mechanisms of the initial biological responses of bone remodeling and regeneration upon mechanical stimuli.
Publication types
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Research Support, Non-U.S. Gov't
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Retracted Publication
MeSH terms
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Animals
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Cell Differentiation / genetics
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Gene Expression Regulation*
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Hydrostatic Pressure
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Male
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Mechanotransduction, Cellular / genetics*
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Mesenchymal Stem Cells / cytology
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Mesenchymal Stem Cells / metabolism*
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Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors
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Mitogen-Activated Protein Kinase 1 / metabolism
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Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors
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Mitogen-Activated Protein Kinase 3 / metabolism
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Osteogenesis / genetics*
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Phosphorylation
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Rats
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Rats, Sprague-Dawley
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Stress, Mechanical
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Wnt Proteins / genetics*
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Wnt4 Protein
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p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
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p38 Mitogen-Activated Protein Kinases / metabolism
Substances
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Wnt Proteins
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Wnt10b protein, rat
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Wnt4 Protein
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Wnt4 protein, rat
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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p38 Mitogen-Activated Protein Kinases