T- helper 1 and 2 cytokine assay in patients with common variable immunodeficiency

J Investig Allergol Clin Immunol. 2008;18(6):449-53.

Abstract

Background: Common variable immunodeficiency (CVID) is a heterogeneous group of disorders, characterized by decreased immunoglobulin levels in serum and increased susceptibility to recurrent infections, autoimmunity, and malignancy. The pathogenesis of CVID is still unknown.

Objectives: This study was performed to investigate T-helper (T(H)) 1 and 2 cytokine levels in patients with CVID.

Material and methods: Twenty-four cases of CVID were studied. Cytokine levels of interleukin (IL)-2, IL-4, IL-10, and interferon gamma were measured in the serum of the patients and compared with those of healthy controls.

Results: T(H)2 cytokine levels (IL-4 and IL-10) were significantly higher in the patient group than in the controls (median: 64.5 vs 0.0 pg/mL, P=.016 for IL-4 and 321.1 vs. 0.0 pg/mL, P=.001 for IL-10). However, there were no significant differences in T(H)1 cytokines between the 2 groups (median, 116.5 vs. 104.5 pg/mL, P=.22 for IL-2 and 50.5 vs. 42.3 pg/mL, P=.32 for IFN-gamma).

Conclusions: Increased levels of IL-4 and IL-10 could indicate high activation of T(H)2 lymphocytes in this group of patients and consequently supports the concept of a bias towards T(H)2-type responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Child
  • Common Variable Immunodeficiency / blood
  • Common Variable Immunodeficiency / immunology*
  • Cytokines / biosynthesis
  • Cytokines / blood*
  • Female
  • Humans
  • Immunoassay
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / blood
  • Interleukin-10 / biosynthesis
  • Interleukin-10 / blood
  • Interleukin-2 / biosynthesis
  • Interleukin-2 / blood
  • Interleukin-4 / biosynthesis
  • Interleukin-4 / blood
  • Male
  • Th1 Cells / immunology*
  • Th2 Cells / immunology*
  • Young Adult

Substances

  • Cytokines
  • Interleukin-2
  • Interleukin-10
  • Interleukin-4
  • Interferon-gamma