A 3-D high-frequency array based 16 channel photoacoustic microscopy system for in vivo micro-vascular imaging

IEEE Trans Med Imaging. 2009 Aug;28(8):1190-7. doi: 10.1109/TMI.2008.2011899. Epub 2009 Jan 6.

Abstract

This paper discusses the design of a novel photoacoustic microscopy imaging system with promise for studying the structure of tissue microvasculature for applications in visualizing angiogenesis. A new 16 channel analog and digital high-frequency array based photoacoustic microscopy system (PAM) was developed using an Nd:YLF pumped tunable dye laser, a 30 MHz piezo composite linear array transducer, and a custom multichannel receiver electronics system. Using offline delay and sum beamforming and beamsteering, phantom images were obtained from a 6 mum carbon fiber in water at a depth of 8 mm. The measured -6 dB lateral and axial spatial resolution of the system was 100+/-5 microm and 45+/-5 microm, respectively. The dynamic focusing capability of the system was demonstrated by imaging a composite carbon fiber matrix through a 12.5 mm imaging depth. Next, 2-D in vivo images were formed of vessels around 100 mum in diameter in the human hand. Three-dimensional in vivo images were also formed of micro-vessels 3 mm below the surface of the skin in two Sprague Dawley rats.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Algorithms
  • Animals
  • Hand / blood supply
  • Humans
  • Image Processing, Computer-Assisted / instrumentation
  • Image Processing, Computer-Assisted / methods*
  • Microscopy, Acoustic / instrumentation
  • Microscopy, Acoustic / methods*
  • Microvessels / anatomy & histology*
  • Phantoms, Imaging
  • Rats
  • Rats, Sprague-Dawley
  • Skin / blood supply