Abstract
Pro-brain-derived neurotrophic factor (proBDNF) and mature BDNF utilize distinct receptors to mediate divergent neuronal actions. Using new tools to quantitate endogenous BDNF isoforms, we found that mouse neurons secrete both proBDNF and mature BDNF. The highest levels of proBDNF and p75 were observed perinatally and declined, but were still detectable, in adulthood. Thus, BDNF actions are developmentally regulated by secretion of proBDNF or mature BDNF and by local expression of p75 and TrkB.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, N.I.H., Intramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal
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Brain-Derived Neurotrophic Factor / genetics
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Brain-Derived Neurotrophic Factor / immunology
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Brain-Derived Neurotrophic Factor / metabolism*
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Cell Survival / physiology
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Cells, Cultured
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Gene Expression Regulation, Developmental
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Gene Knock-In Techniques
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Hippocampus / cytology
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Mice
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Mice, Mutant Strains
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Neurons / cytology
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Neurons / metabolism*
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Protein Precursors / genetics
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Protein Precursors / immunology
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Protein Precursors / metabolism*
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Receptor, Nerve Growth Factor / metabolism
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Receptor, trkB / metabolism
Substances
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Antibodies, Monoclonal
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Brain-Derived Neurotrophic Factor
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Protein Precursors
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Receptor, Nerve Growth Factor
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brain-derived neurotrophic factor precursor
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Receptor, trkB