Abstract
For more effective DNA triplex formation under neutral conditions, we synthesized triplex-forming oligonucleotides containing 8-thioxodeoxyadenosine (s(8)dA) residues in place of the protonated deoxycytidines required for the third base pairing with DNA duplexes. Consequently, it was found that s(8)dA exhibited much stronger hybridization ability than dC under neutral conditions when four s(8)dA bases were arranged in a consecutive sequence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA / chemistry*
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Deoxyadenosines / chemical synthesis*
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Deoxyadenosines / chemistry
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Molecular Structure
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Oligodeoxyribonucleotides / chemical synthesis*
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Oligodeoxyribonucleotides / chemistry*
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Organothiophosphorus Compounds / chemical synthesis*
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Organothiophosphorus Compounds / chemistry
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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6-N-carbamoyl-8-thioxodeoxyadenosine 3'-phosphoramidite
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8-thioxodeoxyadenosine
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Deoxyadenosines
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Oligodeoxyribonucleotides
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Organothiophosphorus Compounds
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DNA