Abstract
An in vivo assay was developed for long-term analyses of Schwann cells at the single cell level. Schwann cells were isolated from sciatic nerves and labeled with the fluorescent gold label Fluoro-Gold. Cells were then transplanted into severed sciatic nerves of young adult rats. Gold-labeled Schwann cells, identified by double-labeling with S100 antibodies, were observed up to 10 mm away from the transplant site and after 90 and 120 days of survival in vivo.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies
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Cell Movement
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Cell Separation
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Cell Survival
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Cytological Techniques*
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Fluorescent Dyes
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S100 Proteins / immunology
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Schwann Cells / physiology*
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Schwann Cells / transplantation
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Sciatic Nerve / cytology
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Sciatic Nerve / physiology
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Stilbamidines*
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Time Factors
Substances
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2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt
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Antibodies
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Fluorescent Dyes
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S100 Proteins
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Stilbamidines