Abstract
We describe a calorimetric assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA) within 5 h. Microbial heat was calculated in culture with and without cefoxitin. Among 30 genetically distinct clinical isolates, 19/20 MRSA (95%) and 10/10 methicillin-susceptible Staphylococcus aureus (100%) were correctly identified. Microcalorimetry may be useful for rapid MRSA screening.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Calorimetry / methods*
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Cefoxitin / pharmacology
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Humans
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Methicillin Resistance*
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Microbial Sensitivity Tests / methods
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Sensitivity and Specificity
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Staphylococcal Infections / microbiology*
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / isolation & purification
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Time Factors
Substances
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Anti-Bacterial Agents
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Cefoxitin