The translation initiation factor DAP5 is a regulator of cell survival during mitosis

Cell Cycle. 2009 Jan 15;8(2):204-9. doi: 10.4161/cc.8.2.7384. Epub 2009 Jan 10.

Abstract

Initiation of protein translation is tightly regulated by various physiological signals and involves cap-dependent and independent mechanisms. DAP5 protein is an eIF4G family member previously implicated in mediating cap-independent IRES driven translation in response to various cellular stresses. Unexpectedly, we have recently found that DAP5 is also essential for continuous cell survival in non-stressed cells. We reported in this respect that the knock down of endogenous DAP5 by RNA-interference induces M-phase specific caspase-dependent cell death. Bcl-2 and CDK1 were identified as DAP5 mRNA targets, the translation of which was selectively reduced in the DAP5 knock down cells. They each possess a functional IRES element in their 5'UTR. Here we review the major results of this study and present new data on the link of DAP5 to additional Bcl-2 family members. In addition we discuss other possible cellular phenotypes resulting from the knock down of DAP5 in these cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • CDC2 Protein Kinase / metabolism
  • Cell Survival / genetics
  • Cell Survival / physiology
  • Eukaryotic Initiation Factor-4G / metabolism*
  • HeLa Cells
  • Humans
  • Mitosis*
  • Peptide Chain Initiation, Translational / genetics
  • Proto-Oncogene Proteins c-bcl-2 / metabolism

Substances

  • EIF4G2 protein, human
  • Eukaryotic Initiation Factor-4G
  • Proto-Oncogene Proteins c-bcl-2
  • CDC2 Protein Kinase