Analysis of the 5' non-coding region of rat liver S-adenosylmethionine synthetase mRNA and comparison of the Mr deduced from the cDNA sequence and the purified enzyme

FEBS Lett. 1991 Sep 23;290(1-2):142-6. doi: 10.1016/0014-5793(91)81245-4.

Abstract

A 3 kb cDNA coding for rat liver S-adenosylmethionine (AdoMet) synthetase has been isolated. The Mr of the protein has been unequivocally determined by cDNA sequencing and enzyme purification on a thiopropyl-Sepharose column. The length of the mRNA 5' non-coding region has been defined by primer-extension analysis. The rat liver cloned cDNA has been also used to detect S-adenosylmethionine synthetase mRNA in human liver.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • Gene Expression
  • Methionine Adenosyltransferase / chemistry
  • Methionine Adenosyltransferase / genetics*
  • Molecular Sequence Data
  • Molecular Weight
  • RNA, Messenger / genetics
  • Rats
  • Restriction Mapping

Substances

  • RNA, Messenger
  • Methionine Adenosyltransferase

Associated data

  • GENBANK/S55977
  • GENBANK/S55979
  • GENBANK/S55982
  • GENBANK/S57635
  • GENBANK/S57637
  • GENBANK/X58288
  • GENBANK/X58289
  • GENBANK/X58801
  • GENBANK/X58802
  • GENBANK/X58803
  • GENBANK/X60882