Macrophage depletion in hypertensive rats accelerates development of cardiomyopathy

J Cardiovasc Pharmacol Ther. 2009 Mar;14(1):68-75. doi: 10.1177/1074248408329860. Epub 2009 Jan 22.

Abstract

Inflammation contributes to the process of ventricular remodeling after acute myocardial injury. To investigate the role of macrophages in the chronic process of cardiac remodeling, they were selectively depleted by intravenous administration of liposomal clodronate in heart failure-prone hypertensive Ren-2 rats from the age of 7 until 13 weeks. Plain liposomes were used for comparison. Liposomal clodronate treatment reduced the number of blood monocytes and decreased the number of macrophages in the myocardium. Compared to plain liposomes, liposomal clodronate treatment rapidly worsened left ventricular ejection function in hypertensive rats. Liposomal clodronate- treated Ren-2 rat hearts showed areas of myocyte loss with abundant inflammatory cell infiltration, predominantly comprising CD4 positive T lymphocytes. The current study showed that lack of macrophages was associated with earlier development of myocardial dysfunction in hypertensive rats. Modulation of macrophage function may be of value in the evolution of cardiomyopathy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology
  • Cardiomyopathies / immunology*
  • Cardiomyopathies / physiopathology
  • Clodronic Acid / administration & dosage
  • Disease Models, Animal
  • Disease Progression
  • Hypertension / complications
  • Hypertension / immunology*
  • Hypertension / physiopathology
  • Injections, Intravenous
  • Liposomes
  • Macrophages / drug effects
  • Macrophages / immunology*
  • Male
  • Monocytes / immunology
  • Myocardium / immunology*
  • Myocardium / pathology
  • Rats
  • Rats, Sprague-Dawley
  • Rats, Transgenic
  • Renin / genetics
  • Stroke Volume
  • Time Factors
  • Ventricular Function, Left
  • Ventricular Remodeling

Substances

  • Liposomes
  • Ren2 protein, mouse
  • Clodronic Acid
  • Renin