Abstract
To characterize the biological features of side population (SP) in oral squamous cell carcinoma (OCC), SP and non-SP were sorted and compared. The SP cells were more clonogenic and in nude mice, only 10,000 SP cells were needed for tumor development compared to 1,000,000 non-SP cells. The SP cells expressed higher levels of ABCG2, ABCB1, CD44, Oct-4, Bmi-1, NSPc1 and CK19. The SP cells generated SP and non-SP populations, whereas the non-SP cells generated only non-SP. These findings provide the first evidence that SP in OCC possesses tumor stem cell phenotypes and may play an important role in OCC tumorigenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Differentiation / metabolism
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Carcinoma, Squamous Cell / metabolism
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Carcinoma, Squamous Cell / pathology*
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Cell Cycle / physiology
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Cell Line, Tumor
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Cell Proliferation
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Cell Transformation, Neoplastic / pathology*
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Head and Neck Neoplasms / metabolism
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Head and Neck Neoplasms / pathology*
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Humans
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Neoplasm Proteins / metabolism
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Neoplasm Transplantation
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Neoplastic Stem Cells / metabolism
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Neoplastic Stem Cells / pathology*
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Transplantation, Heterologous
Substances
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Antigens, Differentiation
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Neoplasm Proteins