Identification of stable S-adenosylmethionine (SAM) analogues derivatised with bioorthogonal tags: effect of ligands on the affinity of the E. coli methionine repressor, MetJ, for its operator DNA

Org Biomol Chem. 2009 Feb 21;7(4):635-8. doi: 10.1039/b816495a. Epub 2008 Dec 19.

Abstract

The efficient synthesis of a range of stable SAM mimetics, and their ability to promote the binding of the E. coli methionine repressor (MetJ) to its operator DNA, is described.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / metabolism*
  • DNA / metabolism*
  • Escherichia coli Proteins
  • Ligands
  • Protein Binding / drug effects
  • Repressor Proteins / metabolism*
  • S-Adenosylmethionine / analogs & derivatives*
  • S-Adenosylmethionine / chemical synthesis

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • Ligands
  • Repressor Proteins
  • methionine repressor protein, Bacteria
  • S-Adenosylmethionine
  • DNA